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Western blotting results were similar to the mRNA levels, as protein levels of Wnt3a, Wnt5a, frizzled 7, disheveled 2, β-catenin (cytoplasmic and nuclear) and LEF1 were significantly increased in the B50, B100 and Minoxidil-treated groups compared with the control and B + IWR-1 groups, whereas the level of GSK3β protein was significantly decreased in the B50, B100 and Minoxidil-treated groups compared with the control and B + IWR-1 groups (Fig

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Simultaneously, oxidative modifications of antioxidant enzymes reduce their catalytic efficiency, creating a feed-forward loop of oxidative injury (45, 88)